# Protocol library

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The controlled documents that define calibration, cultivation, sampling, analysis and replication before results are interpreted.

In brief

The controlled documents that define calibration, cultivation, sampling, analysis and replication before results are interpreted.

## Principle

A protocol is published and versioned before the corresponding result is interpreted. Any substantive change creates a new protocol version.

 |
 Protocol | Purpose | Current state

 | FR-PRO-001 | Peristaltic pump gravimetric calibration | documented; execution pending

 | FR-PRO-002 | Empty-system environmental baseline | method defined

 | FR-PRO-003 | Reference cultivation cycle | crop-specific finalisation

 | FR-PRO-004 | Rotation and matched-control validation | method defined

 | FR-PRO-005 | Sensory discrimination and descriptive analysis | standards-aligned design

Calibration and sensory methods use explicit metrology and sensory-analysis terminology. [R37] [R38] [R39] [R40] [R41] [R48] [R49]

## Required protocol sections

- Research question and preregistered hypothesis.
- Experimental unit, sample size and allocation method.
- System, crop, cultivar and biological-material identifiers.
- Independent, dependent and controlled variables with units.
- Calibration prerequisites and equipment register.
- Time-indexed procedure, sampling and harvest rules.
- Deviation, exclusion and stopping rules.
- Planned statistical analysis and publication criteria.

A protocol receives a permanent identifier before execution. A method change produces a new version; it does not silently overwrite the executed method.

## References

- [R37] Bean, Vern E.; Espina, Pedro I.; Wright, John D.; Sheckels, Sherry D.; Johnson, Aaron N. (2006). NIST Calibration Services for Liquid Volume. *NIST Special Publication 250-72*. https://doi.org/10.6028/NIST.SP.250-72
- [R38] Joint Committee for Guides in Metrology (2008). Evaluation of measurement data — Guide to the expression of uncertainty in measurement. *JCGM 100:2008*. https://doi.org/10.59161/JCGM100-2008E
- [R39] Joint Committee for Guides in Metrology (2012). International vocabulary of metrology — Basic and general concepts and associated terms. *JCGM 200:2012*. https://doi.org/10.59161/JCGM200-2012
- [R40] International Organization for Standardization (2007). ISO 8589:2007 Sensory analysis — General guidance for the design of test rooms. *ISO*.
- [R41] International Organization for Standardization (2016). ISO 13299:2016 Sensory analysis — Methodology — General guidance for establishing a sensory profile. *ISO*.
- [R48] International Organization for Standardization (2008). ISO 5492:2008 Sensory analysis — Vocabulary. *International Standard; Amendment 1 published 2016*.
- [R49] International Organization for Standardization (2023). ISO 8586:2023 Sensory analysis — Selection and training of sensory assessors. *International Standard*.