{"@context":"https://schema.org","@type":"TechArticle","id":"PR-ROT-001","slug":"rotation-validation","canonical_url":"https://flavorotor.com/research/rotation-validation","machine_readable_url":"https://flavorotor.com/research/data/chapters/rotation-validation.json","markdown_url":"https://flavorotor.com/research/markdown/rotation-validation","title":"Rotation measurement and biological comparison","description":"Encoder, accelerometer, light and root-zone measurements used to compare rotating and matched static plants.","chapter":"Rotation and gravity","version":"2.0","updated":"2026-07-29","table_of_contents":[{"id":"mechanical-record","label":"Mechanical record"},{"id":"exposure-record","label":"Plant exposure record"},{"id":"plant-response","label":"Plant response"},{"id":"reporting","label":"Reporting"}],"html":"<h2 id=\"mechanical-record\">Mechanical record</h2>\n<p>The rotation record contains commanded and measured speed, angular position, direction, acceleration and stop duration. A three-axis accelerometer fixed at the plant module records vibration and transient acceleration. Each test reports mean speed, speed ripple, peak acceleration, RMS vibration and the difference between commanded and measured angle.</p>\n<div class=\"table-wrap\"><table><thead><tr><th>Measurement</th><th>Method</th><th>Reported value</th></tr></thead><tbody>\n<tr><td>Angular speed</td><td>encoder count divided by elapsed time</td><td>mean, SD, minimum and maximum</td></tr>\n<tr><td>Angular position</td><td>encoder index at each timestamp</td><td>position error and missed counts</td></tr>\n<tr><td>Acceleration ramp</td><td>encoder and accelerometer time series</td><td>ramp duration and peak acceleration</td></tr>\n<tr><td>Vibration</td><td>three-axis accelerometer at the plant module</td><td>axis-specific RMS and peak acceleration</td></tr>\n<tr><td>Endurance</td><td>loaded continuous run</td><td>temperature, stalls, slip events and speed drift</td></tr>\n</tbody></table></div>\n<h2 id=\"exposure-record\">Plant exposure record</h2>\n<p>Mechanical measurements are synchronised with PPFD, air velocity and root-zone contact. One plant-position record therefore identifies the gravity direction in plant coordinates, incident light, immersion state and local air movement at the same time. This prevents a response caused by light or root-zone exposure from being assigned to rotation alone.</p>\n<div class=\"table-wrap\"><table><thead><tr><th>Control group</th><th>Matched variables</th><th>Difference retained</th></tr></thead><tbody>\n<tr><td>Static control</td><td>crop, cultivar, age, mean PPFD, DLI and root-zone exposure</td><td>no periodic reorientation</td></tr>\n<tr><td>Time-varying-light control</td><td>light sequence and root-zone exposure</td><td>static plant orientation</td></tr>\n<tr><td>Matched-vibration control</td><td>measured vibration spectrum and cultivation conditions</td><td>no drum rotation</td></tr>\n<tr><td>Rotating treatment</td><td>cultivation conditions and sampling schedule</td><td>periodic orientation cycle</td></tr>\n</tbody></table></div>\n<h2 id=\"plant-response\">Plant response</h2>\n<p>Root-tip angle, shoot curvature and elongation are measured from indexed image sequences. Growth, root architecture and plant chemistry are analysed as separate endpoints. The experimental unit is one plant or one independent cultivation cycle; repeated frames from the same plant are not counted as independent biological replicates. Gravitropic interpretation follows the measured angle-time history and the known statolith–auxin response of roots and shoots. <button aria-label=\"Open source record R21\" class=\"research-source-trigger\" data-research-source=\"R21\" type=\"button\">[R21]</button> <button aria-label=\"Open source record R63\" class=\"research-source-trigger\" data-research-source=\"R63\" type=\"button\">[R63]</button></p>\n<h2 id=\"reporting\">Reporting</h2>\n<p>The report publishes the complete speed profile, acceleration trace, light and immersion records, sample count, biological replicate count and analysis code. Effect estimates are reported with confidence intervals. A rotation setting is identified by drum speed, direction, radius, ramp profile, operating duration and stop schedule rather than by a device preset name.</p>","text":"Mechanical record The rotation record contains commanded and measured speed, angular position, direction, acceleration and stop duration. A three-axis accelerometer fixed at the plant module records vibration and transient acceleration. Each test reports mean speed, speed ripple, peak acceleration, RMS vibration and the difference between commanded and measured angle. Measurement Method Reported value Angular speed encoder count divided by elapsed time mean, SD, minimum and maximum Angular position encoder index at each timestamp position error and missed counts Acceleration ramp encoder and accelerometer time series ramp duration and peak acceleration Vibration three-axis accelerometer at the plant module axis-specific RMS and peak acceleration Endurance loaded continuous run temperature, stalls, slip events and speed drift Plant exposure record Mechanical measurements are synchronised with PPFD, air velocity and root-zone contact. One plant-position record therefore identifies the gravity direction in plant coordinates, incident light, immersion state and local air movement at the same time. This prevents a response caused by light or root-zone exposure from being assigned to rotation alone. Control group Matched variables Difference retained Static control crop, cultivar, age, mean PPFD, DLI and root-zone exposure no periodic reorientation Time-varying-light control light sequence and root-zone exposure static plant orientation Matched-vibration control measured vibration spectrum and cultivation conditions no drum rotation Rotating treatment cultivation conditions and sampling schedule periodic orientation cycle Plant response Root-tip angle, shoot curvature and elongation are measured from indexed image sequences. Growth, root architecture and plant chemistry are analysed as separate endpoints. The experimental unit is one plant or one independent cultivation cycle; repeated frames from the same plant are not counted as independent biological replicates. Gravitropic interpretation follows the measured angle-time history and the known statolith–auxin response of roots and shoots. [R21] [R63] Reporting The report publishes the complete speed profile, acceleration trace, light and immersion records, sample count, biological replicate count and analysis code. Effect estimates are reported with confidence intervals. A rotation setting is identified by drum speed, direction, radius, ramp profile, operating duration and stop schedule rather than by a device preset name.","source_ids":["I02","R21","R63"],"visuals":[],"sources":[{"id":"I02","authors":"FlavoRotor project team","year":2026,"title":"FlavoRotor v2.0 system architecture","publication":"Internal engineering design report","doi":null,"url":"/research/platform","source_type":"internal primary record","relevance":"Documents the proposed magnetic drive, axial lighting, four-channel peristaltic dosing and imaging architecture.","verification":"Derived from the original FlavoRotor v2.0 engineering document","verified_on":"2026-07-26","verification_status":"INTERNAL PRIMARY RECORD","verified_against":"Original internal project file and extracted media"},{"id":"R21","authors":"Chehab, E. Wassim; Eich, Elizabeth; Braam, Janet","year":2009,"title":"Thigmomorphogenesis: a complex plant response to mechano-stimulation","publication":"Journal of Experimental Botany","doi":"10.1093/jxb/ern315","source_type":"peer-reviewed review","relevance":"Establishes the biological basis for measuring plant responses to repeated mechanical stimulation.","verification":"Publisher, PubMed, ISO or official proceedings metadata checked 2026-07-26","url":"https://doi.org/10.1093/jxb/ern315","verified_on":"2026-07-26","verification_status":"DOI METADATA CHECKED","verified_against":"Publisher, DOI landing page, PubMed or official repository where available"},{"id":"R63","authors":"Nakamura, Moritaka; Nishimura, Takeshi; Morita, Miyo Terao","year":2019,"title":"Gravity sensing and signal conversion in plant gravitropism","publication":"Journal of Experimental Botany, 70(14), 3495–3506","doi":"10.1093/jxb/erz158","url":"https://doi.org/10.1093/jxb/erz158","source_type":"peer-reviewed review","relevance":"Explains amyloplast sedimentation, gravity signalling, directional auxin transport and differential growth in roots and shoots after reorientation.","verification":"Oxford Academic article metadata, abstract and mechanism sections checked 2026-07-29","verified_on":"2026-07-29","verification_status":"DOI AND PRIMARY PUBLISHER RECORD CHECKED","verified_against":"Oxford Academic, Journal of Experimental Botany"}]}
