A complete channel-specific procedure for converting motor commands into measured liquid volume with uncertainty and drift.
Each pump dispenses into a vessel on a calibrated balance. Mass gain is converted to volume using fluid density at the measured temperature. The test is repeated across channel, dose, speed, tube condition and hydraulic head.
\nThe gravimetric chain follows traceable liquid-volume and uncertainty principles: balance performance, test-liquid density, evaporation, timing, repeatability and calibration state are recorded. Large published peristaltic-pump datasets show why repeated measurements and drift analysis are necessary, but their performance values are not transferred to FlavoRotor.
\n| Factor | Levels |
|---|---|
| Channel | 1, 2, 3, 4 |
| Rotor speed | 5, 15, 30 and 60 rev/min |
| Commanded dose | 0.5, 1, 2, 5 and 10 mL |
| Repetitions | minimum 20 per primary condition |
| Fluid | deionised water and each representative stock class |
| Tube state | new, mid-life and replacement threshold |
| Hydraulic condition | minimum, nominal and maximum inlet head; installed outlet path |
| Direction | forward; reverse purge characterised separately |
Repeatability, bias, residual analysis and method precision are reported using declared statistical procedures rather than a single R² value.
\n| Metric | Gate for initial reservoir dosing |
|---|---|
| Relative bias | ≤ ±3% for doses ≥1 mL within the declared range |
| Repeatability | CV ≤2% for doses ≥1 mL |
| Channel model | residual structure absent and R² reported, not used alone |
| Drift | ≤5% before recalibration or tube replacement |
| Cross-channel contamination | none detected above method limit |
| Backflow/siphon | no uncontrolled transfer in the installed hydraulic range |
These are FlavoRotor acceptance criteria, not claimed achieved performance. Published multi-channel pump data guide the method but are not copied as FlavoRotor results.
\nThe system-specific configuration on this page is traced to the supplied FlavoRotor engineering records.
","text":"In brief A complete channel-specific procedure for converting motor commands into measured liquid volume with uncertainty and drift. Principle Each pump dispenses into a vessel on a calibrated balance. Mass gain is converted to volume using fluid density at the measured temperature. The test is repeated across channel, dose, speed, tube condition and hydraulic head. C-1 V i = (m after,i − m before,i ) / ρ(T) Delivered volume for repetition i. C-2 Q i = V i / Δt i Mean flow for repetition i. The gravimetric chain follows traceable liquid-volume and uncertainty principles: balance performance, test-liquid density, evaporation, timing, repeatability and calibration state are recorded. Large published peristaltic-pump datasets show why repeated measurements and drift analysis are necessary, but their performance values are not transferred to FlavoRotor. [R37] [R38] [R47] Test matrix Factor Levels Channel 1, 2, 3, 4 Rotor speed 5, 15, 30 and 60 rev/min Commanded dose 0.5, 1, 2, 5 and 10 mL Repetitions minimum 20 per primary condition Fluid deionised water and each representative stock class Tube state new, mid-life and replacement threshold Hydraulic condition minimum, nominal and maximum inlet head; installed outlet path Direction forward; reverse purge characterised separately Calibration statistics C-3 V̄ = (1/N) ΣV i Mean delivered volume. C-4 bias = V̄ − V set Absolute systematic error at a test point. C-5 CV = 100 · s / V̄ Coefficient of variation for repeatability. C-6 RMSE = √[(1/N)Σ(V i − V set )²] Combined deviation from the requested volume. Repeatability, bias, residual analysis and method precision are reported using declared statistical procedures rather than a single R² value. [R46] [R50] Channel model C-7 V̂ j = a j N cmd + b j First candidate model for channel j; residuals determine whether speed, pressure or nonlinear terms are required. Predefined engineering acceptance gates Metric Gate for initial reservoir dosing Relative bias ≤ ±3% for doses ≥1 mL within the declared range Repeatability CV ≤2% for doses ≥1 mL Channel model residual structure absent and R² reported, not used alone Drift ≤5% before recalibration or tube replacement Cross-channel contamination none detected above method limit Backflow/siphon no uncontrolled transfer in the installed hydraulic range These are FlavoRotor acceptance criteria, not claimed achieved performance. Published multi-channel pump data guide the method but are not copied as FlavoRotor results. [R19] FlavoRotor design provenance The system-specific configuration on this page is traced to the supplied FlavoRotor engineering records. [I03]","source_ids":["I03","R19","R37","R38","R46","R47","R50"],"visuals":[],"sources":[{"id":"I03","authors":"FlavoRotor project team","year":2026,"title":"FlavoRotor peristaltic pump technical record","publication":"Internal engineering record","doi":null,"url":"/research/peristaltic-pump","source_type":"internal primary record","relevance":"Documents CAD geometry, components, first-order equations, four-channel integration and the proposed calibration protocol.","verification":"Derived from the original FlavoRotor pump engineering package","verified_on":"2026-07-26","verification_status":"INTERNAL PRIMARY RECORD","verified_against":"Original internal project file and extracted media"},{"id":"R19","authors":"Buchhorn, Michael; Akkoc, Gun Deniz; Dworschak, Dominik","year":2025,"title":"An open-source peristaltic pump with multiple independent channels for laboratory automation","publication":"Digital Discovery","doi":"10.1039/D5DD00157A","source_type":"peer-reviewed engineering research","relevance":"Provides a relevant multi-channel open-source pump architecture and a measured calibration methodology.","verification":"Publisher metadata and full text checked 2026-07-26","url":"https://doi.org/10.1039/D5DD00157A","verified_on":"2026-07-26","verification_status":"DOI METADATA CHECKED","verified_against":"Publisher, DOI landing page, PubMed or official repository where available"},{"id":"R37","authors":"Bean, Vern E.; Espina, Pedro I.; Wright, John D.; Sheckels, Sherry D.; Johnson, Aaron N.","year":2006,"title":"NIST Calibration Services for Liquid Volume","publication":"NIST Special Publication 250-72","doi":"10.6028/NIST.SP.250-72","source_type":"official metrology publication","relevance":"Provides traceable liquid-volume calibration concepts relevant to gravimetric pump calibration and uncertainty.","verification":"Official NIST metadata checked 2026-07-26","url":"https://doi.org/10.6028/NIST.SP.250-72","verified_on":"2026-07-26","verification_status":"DOI METADATA CHECKED","verified_against":"Publisher, DOI landing page, PubMed or official repository where available"},{"id":"R38","authors":"Joint Committee for Guides in Metrology","year":2008,"title":"Evaluation of measurement data — Guide to the expression of uncertainty in measurement","publication":"JCGM 100:2008","doi":"10.59161/JCGM100-2008E","source_type":"international metrology guide","relevance":"Defines the framework for measurement models, standard uncertainty, combined uncertainty and expanded uncertainty.","verification":"Official BIPM/JCGM record checked 2026-07-26","url":"https://doi.org/10.59161/JCGM100-2008E","verified_on":"2026-07-26","verification_status":"DOI METADATA CHECKED","verified_against":"Publisher, DOI landing page, PubMed or official repository where available"},{"id":"R46","authors":"Heckert, N. Alan; Filliben, James J.; Croarkin, C. M.; Hembree, B.; Guthrie, William F.; Tobias, P.; Prinz, J.","year":2002,"title":"NIST/SEMATECH e-Handbook of Statistical Methods","publication":"NIST Handbook 151","doi":null,"url":"https://www.nist.gov/publications/handbook-151-nistsematech-e-handbook-statistical-methods","source_type":"official statistical handbook","relevance":"Supports calibration regression, residual analysis, experimental design and statistical process control.","verification":"Official NIST publication record checked 2026-07-26","verified_on":"2026-07-26","verification_status":"OFFICIAL RECORD CHECKED","verified_against":"Named official institution record"},{"id":"R47","authors":"Privitera, Davide; Mecocci, Alessandro; Bartolini, Sandro","year":2025,"title":"Extensive Dataset for Peristaltic Pump Accuracy Enhancement in Pharmaceutical Environments","publication":"Scientific Data, 12, 1618","doi":"10.1038/s41597-025-05902-z","source_type":"peer-reviewed data descriptor","relevance":"Supports repeated gravimetric characterisation, drift monitoring and data-driven compensation for peristaltic dosing.","verification":"Publisher and PubMed metadata checked 2026-07-26","url":"https://doi.org/10.1038/s41597-025-05902-z","verified_on":"2026-07-26","verification_status":"DOI METADATA CHECKED","verified_against":"Publisher, DOI landing page, PubMed or official repository where available"},{"id":"R50","authors":"International Organization for Standardization","year":2025,"title":"ISO 5725-2:2025 Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method","publication":"ISO","doi":null,"url":"https://www.iso.org/standard/90054.html","source_type":"international standard","relevance":"Supports estimation of repeatability and reproducibility of measurement methods.","verification":"Official ISO record checked 2026-07-26; ISO 5725-2:2025 is the current published edition.","verified_on":"2026-07-26","verification_status":"OFFICIAL RECORD CHECKED","verified_against":"Official ISO catalogue record"}]}